Miniaturized Two-photon Microscopy Core Lab

Equipment Name	Transcend Miniature Two-Photon Imaging System
Description	Lasers Spark 920 nm fiber laser Fatonics 1030 nm fiber laser Emission Filters Filter group of Green (520 ± 50 nm) Filter group of Red (625 ± 50 nm) Headpiece Universal headpiece Weight of 2.501 g 3x objective Water/hydraulic gel Resolution (xyz) of 0.843 μm × 0.843 μm × 6.88 μm Working distance of 1058 μm Imaging speed of 19.42 Hz@256 × 220 pixels Field of view of 497.19 μm × 401.82 μm Z-scanning ETL integrated: ~240 μm Large Field headpiece Weight of 2.408 g 1.6x objective Water/hydraulic gel Miniature 3D varifocal unit (zoom range: ~450 μm, response speed of 40 ms) Resolution (xy) of 1.550 μm × 1.550 μm Working distance of 1031.3 μm Imaging speed of 18.64 Hz@256 × 220 pixels Field of view of 920.72 μm × 763.4 μm Wide field microscope Camera: Basler acA1920-50gm Resolution: 1920 × 1200 pixels Field of view: 3446 μm × 2154 μm Frame rate: 30 Hz Behavioral recordings Camera: 2 × Basler acA1600-60gm Frame rate: 25 Hz Resolution: 1600 × 1200 pixels Optogenetic Module 473 nm laser 635 nm laser External trigger ports 2 × SMA
Core Location	Room 209, Lo Kwee-Seong Integrated Biomedical Sciences Building
*Standard Price (per hour)**	HKD 280
Technical Support	Dr. Fei PENG CU Email: feipeng@cuhk.edu.hk Tel: 3943 5315

*Note:

• CAT I – GCNI core faculty members (standard price × 0.25)
• CAT II – GCNI associate faculty members or CUHK full-time staff collaborating with GCNI members (standard price x 0.5)
• CAT III – Other CUHK member (standard price)
• CAT IV – Members from other UGC-funded academic institutions (standard price × 2)